Immunogenecity of a chimeric protein of Bacillus anthracis protective antigen and lethal factor in murine model
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چکیده
منابع مشابه
Antibody titers of PEG-PLA block copolymer nanosphere containing chimeric recombinant protein of protective antigen and lethal factor of Bacillus anthracis
Introduction: To date, many vaccines have been developed for anthrax but not yet an ideal vaccine. In this study, chimeric protein containing domain 1 lethal factor and domain 4 protective antigens of Bacillus anthracis in copolymer nanocapsules were used to solve the problems caused by existing vaccines and to increase the efficiency of the proposed vaccine. Materials and Methods: In this expe...
متن کاملPreparation and Evaluation of Human-Murine Chimeric Antibody against Protective Antigen of Bacillus anthracis
The aim of this research is to develop a human/murine chimeric Fab antibody which neutralizes the anthrax toxin, protective antigen (PA). The chimeric Fab was constructed using variable regions of murine anti-PA monoclonal antibody in combination with constant regions of human IgG. The chimeric PA6-Fab was expressed in E. coli. BL21 and evaluated by ELISA and co-immunoprecipitation- mass spectr...
متن کاملCrystallization of the protective antigen protein of Bacillus anthracis.
The protective antigen protein, one of the three separate proteins constituting the exotoxin system of Bacillus anthracis, has been crystallized in a form suitable for structural studies. The crystal form which is most amenable to x-ray analysis is orthorhombic, space group P2(1)2(1)2(1), a = 101.1 A, b = 95.4 A, c = 87.3 A, with one protective antigen monomer/asymmetric unit. The crystals diff...
متن کاملConstruction of a Eukaryotic Plasmid Encoding Bacillus anthracis Protective Antigen, a Candidate for DNA Vaccine
Background: DNA immunization with plasmid DNA encoding bacterial, viral, parasitic and tumor antigens has been reported to trigger protective immunity. Objective: To evaluate the use of a DNA immunization strategy for protection against anthrax, a plasmid was constructed. Methods: The partialsequence of protective antigen of Bacillus anthracis, amino acids 175-764, as a potent immunogenic targe...
متن کاملIn-silico Design and Antibody Response to the Peptide Sequences from Protective Antigen and Lethal Factor Toxins of Bacillus anthracis
Bacillus anthracis is the only pathogen known to be highly infectious with potential biothreat in the family Bacillaceae. The organism in its endospore form has the potential to get aerosolized and infect large population resulting in panic and mass causality (Wilkening, 2006). Because of this reason, Center for Disease control and prevention (CDC) has listed Bacillus anthracis as category A ag...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2016
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2016.02.906